- Environmental Evaluation
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Field bioassays for early detection of chronic impacts of chemical wastes upon marine organisms
Helgoländer Meeresuntersuchungen volume 33, pages 531–545 (1980)
Abstract
A major problem facing those who must assess the environmental effects of the disposal in the ocean of industrial and municipal wastes, including dredged materials, is determining whether given wastes elicit chronic deteriorative responses in important species of organisms. The full importance of such low-level, nonlethal effects is not known, but it is suspected that repeated elicitations may result in ecosystem changes as important as those caused by more easily determinable acute effects. Such considerations are important to the marine environment, where dumped pollutants may be quickly diluted to legal nonlethal concentrations, but may still bring forth cumulative chronic response patterns. One objective of this study has been to develop a field method of assessing the impacts of the disposal of various industrial and municipal wastes. The measure of the impact is not mortality measured against time, but the increase or decrease in activity of certain metabolic enzymes that signal whether an organism is under stress from a class of wastes. Also, by analysing tissues of test and indigenous species for the accumulation of metals, PCBs, and high molecular weight hydrocarbons as well as for the enzyme activity, one gains an insight into the actual effect, if any, of the accumulation upon the whole organism. The test organisms are exposed for selected periods of time in the field in devices called Biotal Ocean Monitors (BOMs); they are then assayed for enzyme induction. At present the following enzymes are used: mitochondrial ATPase, which responds particularly to excess biphenyls in the environment; catalase that is dissolved in the cytosol and responds to excesses of toxic metals; and cytochrome P-420 and P-450, which respond to cyclic and long-chain hydrocarbons. The applicability of the adenylate energy charge system to this problem is also studied.
Literature cited
Atkinson, D. E., 1969. Regulation of enzyme function. — A. Rev. Microbiol.23, 47–68.
Bend, J. R. & Hook, G. E. R., 1977. Hepatic and extrahepatic mixed function oxidases. In: Handbook of physiology, Sect. 9: Reactions to environmental agents. Ed. by. S. R. Geiger. Waverly Press, Baltimore, 419–440.
Brezonik, P. C., Browne, F. W., & Fox, J. L., 1975. Application of ATP to plankton and bioassay studies. — Wat. Res.9, 155–162.
Buhler, D. R., 1966. Hepatic drug metabolism in fishes. — Fedn Proc. Fedn Am. Socs exp. Biol.25, 343.
Burns, K. A., 1976. Microsomal mixed function oxidases in an estuarine fish,Fundulus heteroclitus, and their induction as a result of environmental contamination. — Comp. Biochem. Physiol.53B, 443–446.
Chambers, J. E. & Yarbrough, J. D., 1976. Xenobiotic biotransformation systems in fishes. — Comp. Biochem. Physiol.55C, 77–84.
Chapman, A. G., Fall, L. & Atkinson, D. E., 1971. Adenylate energy charge inEscherichia coli during growth and starvation. — J. Bact.108, 1072–1086.
Fritz, P. J. & Hamrick, M. E., 1966. Enzymatic analysis of adenosintriphosphatase. — Enzymologia30, 57.
Lidman, U., Forlin, L., Molander, O. & Axelson, G., 1976. Induction of the drug metabolizing system in rainbow trout (Salmo gairdnerii) liver by polychlorinated biphenyls (PCBs). — Acta pharmac. tox.39, 262–272.
Omura, T. & Sato, T., 1964. The carbon monoxide-binding pigment of liver microsomes II. Solubilization, purification, and properties. — J. biol. Chem.239, 2379–2385.
Pardini, R. S., 1971. Polychlorinated biphenyls (PCB): effect on mitochondrial enzyme systems. — Bull. environ. Contam. Toxicol.6, 539.
Pequegnat, W. E., 1978. Combined field-laboratory approaches to detecting impacts of waste materials on aquatic organisms. In: Third annual conference on treatment and disposal of industrial wastewaters and residues, Houston. Information Transfer Inc., Rockville, Md., 6 pp.
Pequegnat, W. E., James, B. M., Kennedy, E. A., Fredericks, A. D. & Fay, R. R., 1978. Development and application of a biotal ocean monitor system to studies of the impact of ocean dumping. — TerEco Corporation Report to U. S. Environmental Protection Agency, 143 pp.
Pequegnat, W. E., James, B. M., Kennedy, E. A., Fredericks, A. D., Fay, R. R. & Hubbard, F., 1979. Application of the biotal ocean monitor system to a preliminary study of the impacts of ocean dumping of dredged material in the New York Bight. — TerEco Corporation Report to U. S. Army Engineer Waterways Experiment Station, Vicksburg, 76 pp.
Pullman, M. E., Penefsky, H. S., Datta, A., & Racker, E., 1960. The assay of ATPase activity. — J. biol. Chem.235, 3322.
Statham, C. N., Elcombe, C. R., Szyjka, S. P. & Lech, J. J., 1978. Effect of polycyclic aromatic hydrocarbons on hepatic microsomal enzymes and disposition of methylnaphthalene in rainbow trout in vivo. — Xenobiotica8, 65–71.
Stegeman, J. J., 1978. Influence of environmental contamination on cytochrome P-450 mixed function oxygenases in fish: Implications for recovery in the Wild Harbor Marsh. — J. Fish. Res. Bd Can.35, 668–674.
U. S. Environmental Protection Agency, 1978. Bioassay procedures for the ocean disposal permit program. Environmental Research Laboratory, Office of Research and Development, U. S. Environmental Protection Agency, Gulf Breeze, Florida 32561,EPA-600/9-78-010, 4–121.
U. S. Environmental Protection Agency & U. S. Army Corps of Engineers, 1977. Ecological evaluation of proposed discharge of dredged material into ocean waters. Implementation manual for Section 103 of Public Law 92-532 (Marine Protection, Research, and Sanctuaries Act of 1972). Environmental Effects Laboratory, U. S. Army Engineer Waterways Experiment Station, Vicksburg, Miss., 84 pp.
Wastler, T. A., Offutt, C. K., Fitzsimmons, C. K. & Des Rosiers, P. E., 1975. Disposal of organochlorine wastes by incineration at sea. Office of Water and Harzardous Materials, Washington, D. C.,EPA-430/9-75-014, 1–226.
Yarbrough, J. D. & Chambers, J. E., 1977. Crude oil effects on microsomal mixed function oxidase system components in the striped mullet (Mugil cephalus). — Life Sci.21, 1095–1100.
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Pequegnat, W.E., Wastler, T.A. Field bioassays for early detection of chronic impacts of chemical wastes upon marine organisms. Helgolander Meeresunters 33, 531–545 (1980). https://doi.org/10.1007/BF02414777
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DOI: https://doi.org/10.1007/BF02414777