Influence of light quality and gassing on the vertical migration of diatoms inhabiting the Wadden Sea

Abstract

Diatoms inhabiting the Wadden Sea show a rhythmic migration pattern, which is superimposed by the tidal rhythm. In addition to light intensity, light quality has a pronounced influence on the upward-directed migration, thus giving some information on the nature of the relevant photoreceptors. Maximum diatom migration occurred when sediment surfaces were illuminated with blue light. The cell densities in blue light exceeded those of white light control experiments 1.8-fold. Furthermore, we registered a minor peak in the red light region, which reached approximately 60% of the white light controls. Cryptochrome and/or phototropin may thus be involved and act as photoreceptors for the vertical migration pattern. Flushing sediment surfaces of freshly mixed Wadden Sea sediments with air, O₂, CO₂ or N₂ did not show a significant influence of O₂ on the upward migration. The disappearance of diatoms from sediment surfaces which were flushed with CO₂ is most probably caused by the acidification of the sediment bed.

Introduction

Most motile diatoms show an endogenous and rhythmic migration pattern (autonomous reversal rhythm): they move back and forth and reverse direction after defined periods of time. Internal and external stimuli modify this migratory behaviour so that it generally becomes visible as a positive or a negative reaction, i.e. migration towards or away from a stimulus (e.g. Wenderoth and IWF 1983). The threshold values which have to be exceeded or fallen below are species-specific (Nultsch 1956; Cohn and Weitzell 1996). Of major importance in diatoms (as photosynthesizing organisms) are light-regulated migratory reactions, i.e. the perception of the direction of the incident light (photo-topotaxis), the reaction induced by a sudden change or a strong gradient in light intensity (photo-phobotaxis) and the influence of light on the speed of movement (photokinesis). The direction of the incident light is perceived by blue light receptors (Nultsch 1971), and blue light receptors and also, in some species, receptors absorbing red light act in photo-phobotaxis (Wenderoth 1979). During photo-topotactic and photo-phobotactic responses, the endogenous rhythmic reversal becomes modified by comparative measurements at both poles of the cell (Wenderoth 1975; Wenderoth and IWF 1984). The relevant receptors for photokinesis, which show maxima in the blue and red light region of spectrum of the visible light, are most probably pigments functioning in photosynthesis (Nultsch 1971). Aerotaxis is one of the non-light-regulated migratory reactions and describes the influence of air on migratory behaviour, generally resulting in either moving towards or away from higher oxygen concentrations (Hopkins 1966; Nultsch 1956).

Most sediment-inhabiting diatoms show a rhythmic vertical migratory behaviour: generally they migrate upward onto the sediment surface with the onset of light, and downward into the sediment bed with incoming tides or when light intensity decreases. As the migratory behaviour persists even under continuous illumination and in complete darkness, an endogenous rhythm seems to be involved (e.g. Harper 1969; Happey-Wood and Jones 1988). In addition to the endogenous rhythm, light intensity and salinity are shown to have a great impact on the vertical migration (Paterson 1986; Sauer et al. 2002). The impacts of light quality and of the availability or lack of air or defined gases have not been investigated in detail. The only data available for diatoms inhabiting the Wadden Sea which deals with these topics are given by Hopkins (1966) for Surirella gemma, syn.: Petrodictyon gemma (Ehr.) D.G. Mann. Maximum cell densities were found when the samples were illuminated with blue light. The filters used in these experiments, however, showed rather large half band widths. The author furthermore found the highest numbers of surfacing cells in the absence of light and air, and the lowest numbers in the presence of both of these factors.

The current work focuses on these topics and tries to answer the question whether diatom populations consisting of many species and taken from natural Wadden Sea sediments show the same responses towards light quality and the availability of specified gases. Diatom populations were taken from intertidal sediments and cultured under almost natural conditions in a laboratory tidal micro-ecosystem prior to the experiments in which the quality of light or the availability of air was altered.

Methods

Field site and sampling conditions

Field samples were collected from muddy intertidal sediments located on the North Sea shore near the harbour of Dangast (Germany) on 23 August 2001. Low tides were at 05:00 and 17:08 and high tides at 11:01 and 23:30 hours. Sampling was performed as described by Sauer et al. (2002). After transfer to the laboratory, the Petri dishes were stored at 20°C under a 12:12 h low white light: dark regime (photon-fluence rate10 µmol m⁻² s⁻¹ during the light phase provided by Osram L36/32 Luminux Warmton fluorescence tubes in combination with Osram L36/16 Tageslicht fluorescence tubes) in a laboratory tidal micro-ecosystem for up to 21 days. The micro-ecosystem is shown in Fig. 1. Tidal cover and tidal exposure were reached by a motor-driven rake which moved up and down in a tank filled with 20 l artificial seawater medium at defined times. The Petri dishes were placed onto the rake and notched so that the water could drain off completely. The rake moved approximately 2–5 cm into the water. Thus, the device only simulated covered and uncovered conditions of the sediments and did not reduce the light quantity significantly. Low tide occurred at 08:30 and 14:30, respectively, and high tide at 20:30 and 02:30 hours. Both were reached within a time period of 6 min. Tropic Marin (Dr. Biener GmbH, Wartenberg, Germany) was used as a salt source for the artificial seawater medium whose salinity was adjusted to 35 ppm.

Fig. 1

Schematic drawing of the laboratory tidal micro-ecosystem. The tank was filled with 20 l artificial seawater. Petri dishes containing Wadden Sea sediment were placed onto a rake which was moved down into or out of the seawater by a timer-controlled motor. The Petri dishes were notched so that the water could drain off completely. Low intensity white light with a photon-fluence rate of 10 µmol m⁻² s⁻¹ was provided from above

Experimental setup

Experiments were set up within the first hour after onset of irradiation, which was at 09:00 hours. Sediment samples were homogeneously mixed with acid-cleaned sea sand (Merck, Darmstadt, Germany) which was pre-wetted with artificial seawater. The slurry was filled into small Petri dishes of 30 mm diameter (7 g sediment per Petri dish) and incubated as described below.

Light quality experiments

In a first set of experiments (n=3 for each wavelength investigated), the Petri dishes were kept at 18°C for 3 h either in complete darkness or exposed to light wavelengths of 410, 424, 432, 443, 452, 461, 476, 487, 493, 503, 512, 525, 537, 545, 564, 578, 585, 595, 615, 627, 646, 661, 669, 680, 700 or 715 nm (see Fig. 2) which were generated by placing interference filters (Schott, Mainz, Germany, half band width 10 nm) in the slide stages of projectors (Leitz, Wetzlar, Germany). The photon-fluence rates were adjusted to 0.5, 1.0, 2.5 and 5.0 µmol m⁻² s⁻¹, for each wavelength by increasing or reducing the light intensity of the projector and/or placing neutral glass filters (Schott, Mainz, Germany) onto the Petri dishes. Incubation of Petri dishes under white light of 5.0 µmol m⁻² s⁻¹ was used as a positive control for upward directed vertical migration. Then, the cell densities of diatoms that had surfaced were documented by epifluorescence light microscopy.

Fig. 2

Experimental setup of the light quality experiments. Interference filters of wavelengths ranging from 410 to 715 nm were placed in the slide stages of projectors. The photon-fluence rates were adjusted to 0.5, 1.0, 2.5 and 5.0 µmol m⁻² s⁻¹. Incubation of Petri dishes under white light with a photon-fluence rate of 5.0 µmol m⁻² s⁻¹ was used as a positive control for upward vertical migration (white Petri dish)

Gas flushing experiments

The second set of experiments (n=3) focused on the influence/availability of gases on the upward vertical migration. The experimental setup is shown in Fig. 3. Either air delivered by a small aquarium pump or O₂, N₂, CO₂ from gas cylinders were used. The gases were pre-wetted by guiding them through a washing flask filled with water prior to flushing. The sediments were poured into small Petri dishes and placed without lids in glass chambers with in- and outlet ports for the gases. The glass chambers were kept at 20°C for 3 h and either illuminated with white light of 200 µmol m⁻² s⁻¹ intensity or kept in the dark.

Fig. 3

Experimental setup of the gas flushing experiments. Either air provided by an aquarium pump or O₂, N₂, CO₂ from gas cylinders were pre-wetted by guiding them through a washing flask filled with water prior to flushing. The sediments were poured into Petri dishes and placed without lids in glass chambers with in- and outlet ports for the gases. The glass chambers were kept at 20°C for 3 h and either illuminated with white light of a photon-fluence rate of 200 µmol m⁻² s⁻¹ or kept in the dark (not shown in this figure)

Fluorescence microscopy

Cell densities on the surfaces of large sediment areas within the Petri dishes were examined using epifluorescence light microscopy as described by Sauer et al. (2002). At least 30 pictures were taken per Petri dish and stored as digitized files on a personal computer. The diatoms, i.e. their chlorophyll fluorescence, were counted directly from the pictures on the screen of the computer using the UTHSCSA Image Tool software. At least 100 pictures originating from the three individual experiments were counted and used as a data set for each wavelength, while approx. 1,000 pictures originating from the three individual flushing experiments were evaluated.

Light measurements and statistical analyses

Measurements of the photon-fluence rates and statistical analyses (t test) were performed as described by Sauer et al. (2002).

Results

Influence of the light quality on the upward directed migration

Diatoms from Wadden Sea sediment samples were homogeneously mixed with pre-wetted sea sand, filled into Petri dishes and allowed to surface for 3 h in the dark, under low intensity white light or under defined light qualities. The number of cells surfacing in white light was used as an internal control for each experiment and its value was set to 100%.

In order to rule out saturation with light in our experiments, the sediment samples were illuminated with the different light qualities at rather low photon-fluence rates of 0.5, 1.0, 2.5 and 5.0 µmol m⁻² s⁻¹. Photon-fluence rate response curves were generated for each of the wavelengths applied. The photon-fluence rate response curves of some light qualities are given in Fig. 4 and show that the curves were almost linear only at photon-fluence rates higher than 1.0 µmol m⁻² s⁻¹. Therefore, for registering the action spectrum curves, only those data were used which were obtained at photon-fluence rates of 5.0 µmol m⁻² s⁻¹ for the different light qualities.

Fig. 4

Photon-fluence rate response curves of some representative wavelengths. The wavelengths are indicated to the right of each curve. The relative cell density per unit area is expressed as a percentage of the white light control

The three experiments gave rise to similar results which are shown in the representative action spectrum curve of Fig. 5. Maximal cell densities on the sediment surface were registered when illuminating with blue light of 443 nm. The values exceeded those of the white light controls approx. 1.8-fold. Irradiation with green light of 530–540 nm led to a strong decrease in the number of cells that surfaced (approx. 20% of the white light controls). Red light of 680 nm had, in comparison to green light, a positive effect, although less pronounced than blue light, and caused values in cell density of 60% of the white light controls. Illuminating the sediments with light qualities of 476 nm, 512 nm, 595 nm and 627–646 nm resulted in the appearance of minor shoulders and maxima. T test analyses conducted with the data sets obtained for 443 nm, 537 nm and 680 nm confirmed that the values were statistically significant (P<0.00012).

Fig. 5

Action spectrum of the upward vertical migration of diatoms inhabiting the Wadden Sea obtained for a photon-fluence rate of 5 µmol m⁻² s⁻¹. The relative cell density per unit area is expressed as a percentage of the white light control

Influence of flushing the sediments with O2, CO2, N2 and air

The results of the flushing experiments are compiled in Fig. 6. Flushing the sediment surface with air in the absence of light (A/D) did not led to an increase in cell density, and gave rise to values similar to those measured immediately after pouring the sediment samples into the Petri dishes (t₀). Gassing with air in the presence of light (A/L) led to a three- to four-fold increase in cell density. Gassing with O₂ in the presence of light (O₂/L) showed a minor but not significant (P>0.2) positive effect on the upward migration when compared to gassing experiments with air in the presence of light (A/L). The amount of cells surfacing either in the presence of air and light (A/L) or in the presence of O₂ and light (O₂/L), however, were significantly higher than the value found when gassing with nitrogen in the presence of light (N₂/L) (both approx. P=0.04). In contrast, flushing with CO₂ in the presence of light (CO₂/L) led to a significant reduction in the number of cells on the surface (P<0.0001) and even fell below values measured for the t₀ and dark controls. Approximately 12% of the cell density of the white light control (L/L) was measured for gassing with CO₂ in the presence of light.

Fig. 6

Histogram showing the influence of flushing the sediment surfaces with either air (A) or the gasses oxygen (O ₂ ), nitrogen (N ₂ ) or carbon dioxide (CO ₂ ) either in the light (L) or dark (D). The relative cell density per unit area is expressed as a % (±SD) of the control (A/L) which was set to 100% in each experiment and thus is shown without SD. t _o Cell density immediately after mixing the sediment samples

Discussion

Light quality has a pronounced effect on the upward migration of diatom communities. Maximum cell densities were found when the sediment samples were illuminated with blue light of 443 nm. Hopkins (1966) investigated the upward migration of Surirella gemma and found that light of 450–500 nm was most effective and resulted in maximum community densities, exceeding those of the daylight controls approx. 1.4-fold. This value is similar to the 1.8-fold higher value we measured for the entire population of diatoms inhabiting the Wadden Sea, compared to white light controls. Furthermore, wavelengths below 440 nm and above 550 nm resulted in fewer surfacing cells compared to white light. The lowest cell densities were registered at 420 and 620 nm. The filters used in these experiments, however, showed rather large half band widths and this might explain why the minor peaks and shoulders which we registered were not visible in Hopkins’ (1966) investigation. Photo-topotaxis seems to play a major role in the vertical migration of diatoms inhabiting the Wadden Sea in their natural habitat. The relevant photoreceptors are cryptochromes and phototropines: both are flavoproteins and sense UV-A and blue light. Genes encoding cryptochromes and phototropins have been cloned and sequenced from a wide variety of plants (e.g. Batschauer 1998). The function and location of the corresponding gene products within the cell have been the subject of recent investigations. In higher plants, cryptochromes are involved in the regulation of circadian rhythms, the inhibition of hypocotyl elongation, cotyledon expansion and the transition to flowering, while phototropins are involved in phototropism, chloroplast migration and stomatal opening (Malakhov and Bowler 2001; Briggs and Christie 2002). Whole genome sequencing, meanwhile, revealed the presence of genes encoding cryptochromes, phototropins and phytochromes in the centric diatom Thalassiosira pseudonana (Bowler et al. 2003). Furthermore, a cryptochrome gene (CRY1) has been cloned and sequenced from the pennate diatom Phaeodactylum tricornutum (Bowler et al. 2003).

In addition, we registered that red light of 680 nm showed a weak positive effect on the upward migration and resulted in cell density values of approx. 68% of the white light controls. This is in contrast to Bowler et al. (2003), who found that cells of P. tricornutum moved away from red light. The effect might be caused by phytochromes, photoreceptors which absorb red and far red light. Phytochromes have serine/threonine protein kinase activity and become translocated from the cytoplasm to the nucleus in a light-regulated manner. Most of the phytochrome-interacting proteins which have been identified are localized in the nucleus and act within a signal transduction pathway which finally leads to the induction or repression of components localised in the cytoplasm or nuclear (Malakhov and Bowler 2001). Currently, this kind of signal cascade, involved in phototactic movement, is unknown in diatoms. Alternatively, pigments functioning in photosynthesis might be responsible for the minor peak at 680 nm. According to Nultsch (1971), photokinesis directly depends on photosynthesis, and enhancement of the overall photosynthetic activity in red light might lead to enhanced migratory activity. Chlorophylls and carotinoids would function as relevant photoreceptors in this case. Currently, almost no data are available on how the spectral quality of light changes within the uppermost millimetres of Wadden Sea sediment. Kühl and Fenchel (2000) investigated the bio-optical characteristics in an artificial cyanobacterial mat and found that visible light was attenuated more than 100 times more strongly than near infrared light. Furthermore, Underwood (2002) found that the photon-fluence rate dropped from approx. 2500 µmol m⁻² s⁻¹ measured at the sediment surface to values less than 100 µmol m⁻² s⁻¹ at a depth of 200 µm in intertidal silt. Thus, extreme gradients in light are highly likely to occur within the uppermost sediment layers, causing photo-phobotactic responses. The ecological relevance of this photo-phobotactic response might be to stay in the vertical horizon which is best suited for photosynthesis. Phytochromes might play a role in this response and cannot currently be ruled out as photoreceptors functioning in the vertical migratory behaviour of diatoms inhabiting the Wadden Sea.

Flushing the sediment surfaces with O₂ did not give rise to a clear positive or negative result. We registered, within the frame of error, almost similar cell densities when gassing with air or oxygen. Thus, we conclude that aerotaxis does not play a major role in the vertical migration pattern under natural conditions. The effect caused by CO₂ can be explained by lowered pH values, as the pH value of the water in the washing flasks dropped from approx. pH 7 to values of pH 4–5 during flushing with CO₂, while almost constant pH values were measured for the other gases. Humid CO₂ thus may lead to lowered sediment pH values and to an acidification of the uppermost sediment layers. This phenomenon might explain the downward migration and disappearance of diatoms from sediment surfaces on rainy days, as rainwater generally shows pH values in the range of pH 4 to 5, similar to those we measured when flushing with CO₂.