Oxygen consumption rate and Na+/K+-ATPase activity in early developmental stages of the sea urchin Paracentrotus lividus Lam.
© Springer-Verlag and AWI 2011
Received: 24 August 2010
Accepted: 20 June 2011
Published: 7 July 2011
Changes in oxygen consumption rate and Na+/K+-ATPase activity during early development were studied in the sea urchin Paracentrotus lividus Lam. The oxygen consumption rate increased from 0.12 μmol O2 mg protein−1 h−1 in unfertilized eggs to 0.38 μmol O2 mg protein−1 h−1 25 min after fertilization. Specific activity of the Na+/K+-ATPase was significantly stimulated after fertilization, ranging up to 1.07 μmol Pi h−1 mg protein−1 in the late blastula stage and slightly lower values in the early and late pluteus stages.
The common sea urchin Paracentrotus lividus is broadly distributed in the sublittoral zones of the northeast Atlantic, Mediterranean and Adriatic Seas where they are exposed to a wide range of temperature and light conditions. Populations in the Adriatic Sea show strong gonadal growth in winter followed by spawning in spring, and moderate gonadal growth in summer followed by spawning in fall (Tomšić et al. 2010). Low water temperatures and short-day photoperiods enhance reproductive development (Shpigel et al. 2004).
It has been clearly demonstrated that the plasma membrane of unfertilized eggs of Paracentrotus lividus contains a Na/K transport system that is stimulated after fertilization (Ciapa et al. 1984). Na+/K+-ATPase activity accounts for a large portion of the energy demand in the early developmental stages of the purple sea urchin Strongylocentrotus purpuratus and the Antarctic sea urchin Sterechinus neumayeri (Leong and Manahan 1997; Marsh et al. 1999), and increases in enzyme activation and transcription of the α-subunit gene have been verified in the late stage blastula and the gastrula of a variety of sea urchin species (Mitsunaga-Nakatsubo et al. 1992, 1996).
In the present paper, we provide data on the energetics of early developmental stages of the sea urchin Paracentrotus lividus Lam. Since gonads are the marketable product of sea urchins, our study may be useful for cultivation and sustainable exploitation of the species (e.g. in the South Adriatic, Bistrina Bay).
Materials and methods
Fertilization and collection of early developmental stages
Adult sea urchins Paracentrotus lividus (Lamarck 1816) were collected in coastal non-polluted waters of the Bay of Gruž in the vicinity of the city of Dubrovnik (Croatia) during the summer of 2006 and autumn of 2007 and 2008. After induced spawning, individuals were returned to the sea. Freshly collected sea urchins were induced to spawn by injection of 1 ml 1.0 M KCl into the coelomic cavity. Sperm was collected into dry containers and stored at 4°C. Spawned eggs were cleaned from debris by washing through 500-μm mesh and suspended (1 × 106 eggs) in 500 ml filtered seawater (0.2 μm pore size Millipore filters). Before use, sperm was diluted 100-fold with filtered seawater. One ml of this sperm suspension was added to fertilize 1 × 106 eggs. Ten minutes after fertilization, eggs were gently washed through a 45-μm mesh screen and resuspended in 5–10 l seawater. Temperature was maintained at 20 ± 1°C during the experiment. Fertilized eggs were incubated in a culture container at a final concentration ranging from 30 to 250 embryos per ml. The container was put on a slowly moving shaker to keep the embryos in suspension. First, second and third cleavages appeared after 1.5, 2.0 and 3.0 h, respectively. About 12, 18, 24 and 48 h after fertilization, blastulae, gastrulae and early and late stage plutei were collected by filtration through 45-μm mesh, additionally centrifuged at 2,000×g for 15 min and then stored at −80°C until use.
Measurement of oxygen consumption
Oxygen consumption rates of unfertilized and fertilized eggs were measured in a closed Perspex chamber containing 50 ml filtered seawater (Millipore filter size 45 μm) at constant room temperature (20 ± 1°C). Measurements were recorded by a Digital Radiometer O2 analyzer (Copenhagen). The tip of a pO2 electrode (E-5046, Radiometer, Copenhagen) extended through the cover into the chamber. A magnetic stirrer gently rotated in the chamber to maintain a uniform O2 tension. The oxygen electrode was calibrated by bubbling the seawater in the chamber with N2 gas to provide a solution with zero O2 saturation. Before O2 was measured, the oxygen meter scale was adjusted to the actual barometric pressure in the oxygen-saturated medium. A suspension of eggs (470 eggs/ml) was uniformly suspended by magnetic stirring (50 revolutions/min). Unfertilized eggs were inseminated by 0.5 ml suspension of sperm diluted 104-fold immediately before injection into the chamber. An experiment was considered valid when more than 95% of the eggs had a regularly developed fertilization membrane within 2 min. The metabolic inhibitor rotenone (Fluka) was added into the chamber in the final concentration of 4.6 μmol/l. Methodological details of O2 consumption measurement were described earlier (Lucu and Pavičić 1995).
Determination of Na+/K+-ATPase activity
Na+/K+-ATPase activity was determined by a modified method described in detail by Lucu and Flik (1999). Defrozen sea urchin embryos were homogenized (1 g fresh weight/5 ml medium) in ice-chilled hyposmotic medium (hypo) that consisted of 12.5 mM NaCl, 1 mM HEPES, 1 mM DTT and 0.5 mM EDTA. The pH was adjusted by TRIS base at 8.1. Immediately before use, the protease inhibitor aprotinin was added (0.1 ml/100 ml hypo). Embryos were homogenized in a Dounce homogenization device by 80 strokes and then centrifuged at 1,000×g for 15 min. The supernatant was separated, and Na+/K+-ATPase activity was assayed in triplicate by incubating 150 μl of supernatant with 500 μl assay solutions A and E. Assay solution A contained 100 mM NaCl, 12.5 mM KCl, 5.0 mM MgCl2, 0.1 mM EDTA, 3 mM Na2ATP and 15 mM imidazole. The pH was adjusted by HEPES at 7.6. Solution E was composed as A, but KCl was omitted and ouabain (2.0 mM/l) was added. The difference in phosphate released from ATP in solutions A and E reflects the ouabain-sensitive Na+/K+-ATPase activity. After incubation in a thermostated bath at 37°C for 30 min, the reaction was stopped by the addition of 1 ml ice-cold 8.6% trichloracetic acid. Liberated phosphate was quantified spectrophotometrically by the addition of 1 ml of 1.14% (wt/vol) ammonium heptamolybdate dissolved in sulphuric acid, supplemented immediately before use with FeSO4 × 7H2O (4.6 g/50 ml). After 30-min incubation, the change in absorbance at 700 nm was recorded. Total ATPases were quantified by triplicates of 100 μl 1.62 mM Pi Sigma standards in assay solution.
The protein content of homogenates was estimated by the Coomassie Brilliant Blue reagent procedure (Bradford 1976; Bio-Rad Perth, Scotland). The reference standard for protein determination was bovine serum albumin (BSA; 2 mg/ml). Protein concentration was measured spectrophotometrically at 595 nm wavelength.
Calculated values were expressed as means ± SD of two small sample groups to determine whether differences between them are significant (Quattro Pro Computer test). Statistical analyses consisted of paired two-tailed Student t-test. P values of <0.05 were considered significant. Significant differences between treatments were accessed by ANOVA in combination with Turkey test. Normality and homogeneity of variances assumptions were checked (Zar 1999).
Results and discussion
When eggs 25 min after insemination were exposed to 4.6 μmol/l rotenone, oxygen consumption rate returned to the level of unfertilized eggs (Fig. 1). In the presence of rotenone, the perivitelline space and the fertilization membrane were not morphologically malformed, but cleavage was completely blocked and further development of the embryo was inhibited. A marked increase in the oxygen consumption rate upon fertilization was also found for other sea urchin species by Fujiwara et al. (1986) and Selak and Scandella (1987). Rotenone is a highly specific metabolic poison that affects cellular aerobic respiration, blocking mitochondrial electron transport by inhibiting NADH-ubiquinone reductase (Singer and Ramsay 1994). Cellular uptake of oxygen is blocked, and production of cellular energy in the form of nucleoside triphosphates is greatly reduced. The inhibitory effect of rotenone is attributed to irreversible binding and inactivation of complex I of the mitochondrial electron transport chain, thereby inhibiting oxidative phosphorylation. In sea urchin eggs, intracellular Ca2+ of mitochondria is a multisite activator of oxidative phosphorylation. Ca2+ signals and mitochondrial ATP production stimulate embryonic development (Eisen and Reynolds 1985; Rizutto et al. 2000). Mitochondria isolated from unfertilized and fertilized eggs of sea urchins Strongylocentrotus purpuratus exhibit coupled 5′-diphosphate (ADP)-dependent oxidation of flavine- and pyridine-linked substrates. In unfertilized eggs, cytochrome was extracted more efficiently. The toxicity of rotenone has been attributed to the inhibition of cell respiration by blocking the oxidation of nicotinamide adenine dinucleotide (NAD) at complex I of the electron transport chain, thus maintaining a high NADH/NAH+ ratio (Pastorino et al. 1995). This may result in a collapse of mitochondrial proton motive force necessary for ATP production (Lindahl and Oberg 1961).
Early developmental stages have a higher specific rate of energy utilization than adult stages. In the embryonal stages, a substantial fraction of the ATP energy was attributed to Na+/K+-ATPase activity. For the blastula and gastrula stages, 23 and 19% of respective metabolic energy could be accounted to sodium pump activity (Leong and Manahan 1997). About 75% of the metabolism of blastula and gastrula stages can be attributed to a combination of Na+/K+-ATPase activity and protein synthesis. In spite of large differences in the rates of protein synthesis, a relatively constant energy cost per unit mass of proteins synthesized was found during phases of sea urchin development (Pace and Manahan 2006).
Under extreme temperature conditions, Antarctic sea urchins showed an increase in the metabolic rate from blastula to late gastrula stage (Shilling and Manahan 1994). In the early development of these organisms, the activity of the Na+/K+-ATPase required about 40% of the total metabolic energy, maintaining intracellular ionic gradients and generating secondary active transport processes of ions and small organic molecules. The maintenance of ionic gradients in the cells is one of the most energy-consuming processes, expanding to 20–30% of the total energy. For the early developmental stages that are dependent on internal food supplies, this process could be of immense importance (Marsh et al. 1999, 2000).
Similar patterns of Na+/K+-ATPase enzyme activity were found in early developmental stages of the sea urchin Hemicentrotus pulcherrimus (Mitsunaga-Nakatsubo et al. 1992). According to these authors, the RNA encoding Na+/K+-ATPase accumulates in ectodermal cells prior to the formation of the mesenchyme in the blastula stage. The activity increased in the mesenchyme blastula stages and reached its maximum in the gastrula stage (Mitsunaga-Nakatsubo et al. 1996). Northern blot analysis using a fragment of the Hemicentrotus Na+/K+-ATPase α-subunit cDNA revealed that the mRNA is about 4.6 Kb long and is maximally expressed in the mesenchyme blastula and the gastrula stage (Mitsunaga-Nakatsubo et al. 1992).
For late stage plutei, we found that saponin-treated homogenates showed a Na+/K+-ATPase activity that was nearly twice higher than that of untreated homogenates (increase from 0.89 to 1.89 μmol Pi h−1 mg protein−1; N = 6; P < 0.001). Such treatment with a detergent permeabilizes the membranes of intracellular organelles containing the pool of Na+/K+-ATPase activity, thereby allowing for the measurement of its maximum activity (Chibalin et al. 1999).
We thank the Ministry of Science, Education and Sports of the Republic of Croatia for financial support (project: 271-001-1501-0828). Experiments comply with the current laws of the Republic of Croatia.
- Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254PubMedView ArticleGoogle Scholar
- Chibalin AV, Ogimoto G, Pedemonte CH, Pressley TA, Katz AI, Feraille E, Berggren PD, Bertorello AM (1999) Dopamine-induced endocytosis of Na+, K+-ATPase initiated by phosphorylation of Ser-18 in the rat α-subunit and is responsible for the decreased activity in epithelial cells. J Biol Chem 274:1920–1927PubMedView ArticleGoogle Scholar
- Ciapa B, de Rensis G, Girard J, Payan P (1984) Sodium-potassium exchange in sea urchin eggs. I Kinetic and biochemical characterization at fertilization. J Cell Physiol 121:235–242PubMedView ArticleGoogle Scholar
- Eisen A, Reynolds GT (1985) Source and sinks for the calcium released during fertilization of single sea urchin eggs. J Cell Biol 100:1522–1529PubMedView ArticleGoogle Scholar
- Epel D (1990) The initiation of development. Cell Differ Dev 29:1–12PubMedView ArticleGoogle Scholar
- Fujiwara A, Tazawa E, Hino A, Asami K, Yasumasu I (1986) Respiration in eggs of the echinoid, Urechis unicinctus, before and after fertilization. Dev Growth Differ 28:431–442View ArticleGoogle Scholar
- Leong PKK, Manahan DT (1997) Metabolic importance of Na+, K+-ATPase activity during sea urchin development. J Exp Biol 200:881–2892Google Scholar
- Lindahl PE, Oberg KE (1961) The effect of rotenone on respiration and its point of attack. Exp Cell Res 23:228–237PubMedView ArticleGoogle Scholar
- Lucu Č, Flik G (1999) Na+, K+-ATPase and Na/Ca2+ exchange activities in gills of hyperregulating Carcinus maenas. Am J Physiol 276:R490–R499PubMedGoogle Scholar
- Lucu Č, Pavičić D (1995) Role of seawater concentration and major ions in oxygen consumption rate of isolated gills of the shore crab Carcinus mediterraneus Csrn. Comp Biochem Physiol 112A:565–572View ArticleGoogle Scholar
- Marsh AG, Leong PKK, Manahan DT (1999) Energy metabolism during embryonic development and larval growth of an Antarctic sea urchin. J Exp Biol 202:2041–2050PubMedGoogle Scholar
- Marsh AG, Leong PKK, Manahan DT (2000) Gene expression and enzyme activities of the sodium pump during sea urchin development: implications for indices of physiological state. Biol Bull 199:100–107PubMedView ArticleGoogle Scholar
- Mitsunaga-Nakatsubo K, Fujiwara A, Yasumasu I (1992) Change in the activity of Na+, K+-ATPase in embryos of the sea urchin Hemicentrotus pulcherrimus, during early development. Dev Growth Differ 34:379–385View ArticleGoogle Scholar
- Mitsunaga-Nakatsubo K, Yamasaki K, Hatoh-Okazaki M, Kawashita H, Okamura C, Akasaka K, Shimada H, Yasumasu I (1996) cDNA cloning of Na+, K+-ATPase α-subunit from embryos of the sea urchin Hemicentrotus pulcherrimus. Zool Sci 13:833–841PubMedView ArticleGoogle Scholar
- Pace DA, Manahan DT (2006) Fixed metabolic costs for highly variable rates of protein synthesis, developmental stage and state of feeding during sea urchin development. J Exp Biol 209:158–170PubMedView ArticleGoogle Scholar
- Pastorino JG, Wilhelm TJ, Glascott PA Jr, Kocsis JJ, Faber JL (1995) Dexamethasone induces resistance to the lethal consequences of electron transport inhibition in cultured hepatocytes. Arch Biochem Biophys 318:175–181PubMedView ArticleGoogle Scholar
- Rizutto R, Bernardi P, Pozzan T (2000) Mitochondria as all-round players of calcium game. J Physiol 529:37–47View ArticleGoogle Scholar
- Runft LL, Jaffe LA, Mehlmann LM (2002) Egg activation at fertilization: where it all begins. Dev Biol 245:237–254PubMedView ArticleGoogle Scholar
- Selak MA, Scandella CJ (1987) Respiration capacity of mitochondria isolated from unfertilized and fertilized sea urchin eggs. Exp Cell Res 169:369–378PubMedView ArticleGoogle Scholar
- Shilling FM, Manahan DT (1994) Energy metabolism and amino acid transport during early development of Antarctic and temperate echinoderms. Biol Bull 187:398–407View ArticleGoogle Scholar
- Shpigel M, McBride SC, Marciano S, Lupatsch I (2004) The effect of photoperiod and temperature on the reproduction of European sea urchin Paracentrotus lividus. Aquaculture 232:343–355View ArticleGoogle Scholar
- Singer TP, Ramsay RR (1994) The reaction site of rotenone and ubiquinone with mitochondrial NADH dehydrogenase. Biochem Biophys Acta 1187:198–202PubMedView ArticleGoogle Scholar
- Tomšić S, Conides A, Dupĉić-Radić I, Glamuzina B (2010) Growth, size class frequency and reproduction of purple sea urchin Paracentrotus lividus (Lamarck 1816) in Bistrina Bay (Adriatic Sea, Croatia). Acta Adriat 51:67–77Google Scholar
- Zar JH (1999) Biostatistical analysis, 4th edn. Prentice Hall, Upper Saddle RiverGoogle Scholar