Range extension and morphological characterization of rhodolith-forming species (Corallinales, Rhodophyta) from shallow water in the Mexican South Pacific
© Springer-Verlag Berlin Heidelberg and AWI 2014
Received: 31 December 2013
Accepted: 15 July 2014
Published: 29 July 2014
Living rhodolith beds are widely distributed along the Eastern Pacific ocean. Despite their widespread distribution, little is known about the rhodolith-forming species from shallow water in the Mexican South Pacific. Many taxonomic and morphological studies about rhodoliths have been carried out in the Gulf of California, where the forming species belong to the Hapalidiaceae and Corallinaceae families. This paper is the first report on the occurrence of the rhodolith-forming Hapalidiaceae species Lithothamnion muelleri and Phymatolithon repandum at three sites in the Mexican South Pacific. The branch density, maximum length and sphericity were measured for each determined species. Rhodoliths were distributed between 4 and 6 m depth, but differences in the branch density between species and sites were not found. Finally, the present record of L. muelleri fills the gap in the species distribution along the Eastern Pacific ocean, while the record of P. repandum is the first of the species in the region.
KeywordsBranch density Lithothamnion muelleri Phymatolithon repandum Sphericity Taxonomy
Rhodoliths are free-living structures composed mostly (>50 %) of non-geniculate coralline red algae. Rhodoliths have ecological, economic and paleoenviromental importance (Foster 2001). Species that form rhodoliths usually cannot be identified with certainty using only variations in growth form or external morphology. Accurate and reliable identification requires the examination of vegetative and reproductive characters (Harvey and Woelkerling 2007).
Living rhodolith beds are widely distributed along the Eastern Pacific Ocean, and they are particularly abundant in the Gulf of California (Foster 2001). Many taxonomic studies have been carried out in the Gulf of California, where the forming species belong to the Hapalidiaceae and Corallinaceae families (Steller et al. 2009). Little is known about rhodolith-forming species in the Mexican South Pacific. Although there are several papers that report the presence of non-geniculate corallines in the area (i.e., Dawson 1960; León-Álvarez and González-González 1993; León-Tejera and González-González 1993; Mendoza-González and Mateo-Cid 1998; Mateo-Cid and Mendoza-González 2012), only Fragoso and Rodríguez (2002) register the rhodolith-forming species, Lithophyllum frondosum and Spongites fruticulosus, but without describing their morphological features.
As far as it is known, in the Mexican South Pacific, rhodoliths occur in Playa Manzanillo and Playa Carey in Ixtapa-Zihuatanejo, Guerrero and Isla Cacaluta in Huatulco, Oaxaca, Mexico. However, the species composition and descriptive morphological characteristics of the rhodoliths in these places are unknown. The current study aims to describe rhodolith-forming species, with respect to growth form, branch density, maximum length and sphericity at three sites in the Mexican South Pacific.
Materials and methods
The collected material was preserved in 4 % formalin in seawater and entered into the Phycological Herbarium of Universidad del Mar, Oaxaca, Mexico.
From each rhodolith collected, permanent slides were made for optical microscopy following the format of Riosmena-Rodríguez et al. (1999). The morphological and anatomical observations follow Woelkerling (1988). The determination to genera and species level followed descriptions by Dawson (1960), Chamberlain and Irvine (1994), Wilks and Woelkerling (1994, 1995) and Woelkerling (1996). Following the previously cited authors, the diagnostic features used to determine species were for Lithothamnion, the morphology and anatomy of the tetrasporangial conceptacle roof and the localization of old conceptacles according to the rounding vegetative tissue, while for Phymatolithon, the rhodolith growth morphology, anatomy of the tetrasporangial conceptacle roof and camera and localization of old conceptacles according to the rounding vegetative tissue. Type specimens were not examined. Typification data follow Woelkerling (1993). For each determined species, the diameter and length measurements from 30 randomly selected epithallial, subepithallial and vegetative cells were taken. Diameter and height of sporangial chambers and length of tetrasporangia were measured from ten randomly selected structures for each species. All measurements were taken using the software Axio Vision Rel. 4.8 from digital photographs.
The maximum length (L), shortest (S) and intermediate (I) dimensions of all specimens collected were measured with a Vernier caliper (±0.01 mm) to calculate rhodolith sphericity following the protocol of Sneed and Folk (1958). Growth form terminology follows Woelkerling et al. (1993). The branch density was estimated through the number of branches in 1 cm2 randomly located on each rhodolith surface. Five such quadrants were measured on each rhodolith. A rarefaction curve was established to obtain the representative sample size.
Lenormand ex Rosanoff, 1866: 101, pl. 6, figs 8–11 (Fig. 2).
CN (herb. Lenormand); from Western Port Bay, Victoria; collected by W. H. Harvey 1851 (communicated by F. Mueller). Lectotype designated by Woelkerling (1983: 193, figs. 30–33) and Wilks and Woelkerling (1995: 555, fig. 1A).
MEL 588439; L 941.149-249 (communicated by Lenormand) (Wilks and Woelkerling 1995).
Plants are non-geniculate, thallus-forming free-living rhodoliths with lumpy to fructicose growth form (Fig. 2a). Nucleus is of stone, coral or fragments of others rhodoliths. Thallus is pseudoparenchymatous with dorsiventral internal construction. Monomerous thallus construction consists of a single system of branched filaments that forms a core, running more or less parallel to the substratum and a more peripheral region with portions of core filaments or their derivatives that curve outwards toward the thallus surface and terminate in a single layer of epithalial cells. Flared epithallial cells (Fig. 2b) measure 3–8 μm in diameter and 2–4 μm in length. Cellular elongation is below the subepithallial region. Subepithallial initials cells (Fig. 2b) are as long or longer (2.8–5 μm in diameter and 4.8–9.9 μm diameter) than the cells immediately below them and measure 2.6–5.2 μm in diameter y 3.5–7.7 μm in length. Adjacent filaments are joined by cell fusions (Fig. 2c). Pit connections and trichocytes have not been observed.
Multiporate tetrasporangial conceptacles are raised in relation to the surrounding vegetative thallus surface; chambers 106–190 μm high and 220–466 μm in diameter (Fig. 2d, f). Roofs of tetrasporangial conceptacles are composed of 5–6 layers of cells. Filaments bordering pore canals of tetrasporangial conceptacle composed of cells that do not differ in size and shape from cells in other roof filaments. Tetrasporangial conceptacle pores are 7.5–10.5 μm in diameter, surrounded by 6–7 cells (Fig. 2e). Mature tetrasporangia measure 25–50 μm in diameter and 80–120 μm in length, containing four zonated arranged tetraspores (Fig. 2g). Multiporate tetrasporangial conceptacles are raised in relation to the surrounding vegetative thallus surface (Fig. 2h). The presence of senescent conceptacles was located in the central region of the thallus (Fig. 2i). Gametangial samples were not seen.
Material examined: Playa Manzanillo, Ixtapa-Zihuatanejo, Guerrero, Mexico (4–6 m deep, June and October 2012, M1II; Playa Carey Ixtapa-Zihuatanejo, Guerrero, Mexico (4–6 m deep, October 2012, N1V); Isla Cacaluta, Huatulco, Oaxaca, Mexico (10 m deep, May 2012, 21C, 27B).
Comparative summary of information for L. muelleri and P. repandum for different geographical areas
Epithalial cells length (μm)
Epithalial cells diameter (μm)
Tetrasporangial conceptacle chamber height (μm)
Tetrasporangial conceptacle chamber diameter (μm)
Mexican South Pacific
Lumpy to fructicose
Gulf of California
Lumpy to fructicose
Robinson et al. (2013)
Lumpy to fructicose
Robinson et al. (2013)
Espírito Santo State, Brazil
Encrusting to lumpy to fructicose
Amado-Filho et al. (2010)
Crustose to warty
Espírito Santo State, Brazil
Henriques et al. (2011)
Mexican South Pacific
Lumpy to fructicose
Encrusting to warty to fructicose
See Woelkerling (1996: 187,129).
Half Moon Bay, Port Phillip Bay, Vic, Australia (Woelkerling 1996: 189).
TRH, unnumbered; includes slides 358 and 516; designated by Adey in Adey and Lebednik (1967) (Woelkerling 1996: 189).
Plants are non-geniculate, thallus-forming free-living rhodoliths with lumpy to fructicose growth form. Nucleus is of stone, coral or fragments of other rhodoliths. Thallus is pseudoparenchymatous with dorsiventral internal construction. Monomerous thallus construction consists of a single system of branched filaments that forms a core, running more or less parallel to the substratum and a more peripheral region with portions of core filaments or their derivatives that curve outwards toward the thallus surface and terminate in a single layer of epithelial cells. Epithallial cells have a rounded wall (Fig. 3b) that measures 5–12 μm in diameter and 2–5.8 μm in length. Cellular elongation is below the subepithallial region. Subepithallial initial cells (Fig. 3b) are as short or shorter (4.3–9.5 μm in diameter and 2.2–8 μm diameter) as the cell immediately below them that measures 4–11 μm in diameter and 3.9–10.5 μm in length. Adjacent filaments are joined by cell fusions (Fig. 3c). Pit connections and trichocytes have not been observed.
Multiporate tetrasporangial conceptacles are raised in relation to the surrounding vegetative thallus surface; chambers 93–129 μm high and 271–377 μm in diameter (Fig. 3d). Mature tetrasporangia measure 26–33 μm in diameter and 48–53 μm in length, containing four zonated arranged tetraspores (Fig. 3e). Tetrasporangial conceptacle pore is 5–8.6 μm in diameter, surrounded by 6 cells (Fig. 3f). Mature conceptacles are not filled with enlarged irregularly shaped vegetative cells interspersed among the sporangia. Senescent conceptacles were located in central region of thallus (Fig. 3g). Gametangial samples were not seen.
Material examined: Playa Manzanillo, Ixtapa-Zihuatanejo, Guerrero, Mexico (4–6 m deep, June and October 2012, M1I; Playa Carey Ixtapa-Zihuatanejo, Guerrero, Mexico (4–6 m deep, October 2012, N2V, N1II); Isla Cacaluta, Huatulco, Oaxaca, Mexico (10 m deep, May 2012, 27A).
The fructicose rhodolith morphology and the vegetative and reproductive characters measured in the examined specimens agree with characteristics cited for the species in Southern Australia (Woelkerling 1996). According to Woelkerling (1996), the species occurs from Eyre, West Australia to Cape Conran, Victoria, and Tasmania. The species has been recorded in the Western Pacific ocean in the Federated States of Micronesia (Lobban and Tsuda 2003) and Mariana Islands (Tsuda 2003). The present record is the first of this species in the Eastern Pacific ocean.
Rhodolith maximum length and branch density
n = 24
n = 6
n = 9
n = 3
n = 2
n = 3
Maximum length (mm)
Maximum branch density (branches cm2)
Minimum branch density (branches cm2)
The non-geniculate coralline algae L. muelleri and P. repandum are reported for the first time for the Mexican South Pacific. For L. muelleri, the previous records from the Mexican Pacific are from the Gulf of California (Robinson et al. 2013), while for P. repandum, this is the first record of its presence in the Eastern Pacific.
The presence of the genus Lithothamnion in the Mexican South Pacific has been recorded previously, but with the species Lithothamnion phymatodeum Foslie 1902 (Dawson 1960; León-Álvarez and González-González 1993; Fragoso and Rodríguez 2002). The similarities between L. phymatodeum and the present recorded species L. muelleri include a roof in mature tetrasporangial conceptacles not pitted with depressions and the pore canals bordered by cells that do not differ in size and shape from cells in other roof filaments (Dawson 1960; Masaki 1968; Woelkerling 1996; Fragoso and Rodríguez 2002). Nevertheless, the distinguished features reported in L. muelleri and observed in the examined material include the presence of the tetrasporangial roof usually not broken (Fig. 2d) and buried conceptacles (Fig. 2i) (Woelkerling 1996).
Regarding the presence of the genus Phymatolithon in the Mexican South Pacific, the only species reported is P. lenormandii (Areschoug) Adey 1966 (Dawson 1960; Huerta and Tirado 1970; León-Álvarez and González-González 1993; León-Tejera and González-González 1993; Mateo-Cid and Mendoza-González 2012). The particular characteristics cited for P. lenormandii include old conceptacles, not becoming buried (Chamberlain and Irvine 1994), and the absence of protuberances, with usually the thallus having a flat surface (Dawson 1960; Masaki 1968). The cited features were not observed in the studied material Phymatolithon. On the other hand, the characteristics of fructicose thallus and tetrasporangial conceptacle chambers—not filled with large, sterile cells, as reported for P. repandum (Woelkerling 1996)—match with the observed features in the studied specimens (Fig. 3d).
Bastida-Zavala et al. (2013) listed 21 non-geniculate corallines for the central part of the South Mexican Pacific. The present study increases the diversity of the group by 9.5 %; nevertheless, the numbers could increase after examination of new locations, for example in deeper water.
Finally, the rhodoliths generally showed a lower branch density value (1–3.8) in comparison with those reported for shallow water rhodoliths in the Gulf of California by Steller et al. (2003, 2009). The authors related such low values with low to moderate water movement commonly found in current rhodolith beds. Nevertheless, the high percentage of spheroidal rhodoliths found in the present study (Fig. 4) suggest evidence for stronger conditions and shorter waves in contrast to bottom currents (Foster 2001). In order to test this prediction, it will be necessary to increase the number of rhodoliths measured and to measure direct or indirect movement in the water column.
We are grateful to “Programa para el mejoramiento del Profesorado”-PROMEP-2011 for the financial support under the project “Rodolitos en el Pacífico sur de México: especies formadoras, tasa de crecimiento individual e invertebrados asociados”. Also, we thank Dr. Norma A. López-Gómez and Dr. Candelaria F. Candelaria Silva from the Universidad Nacional Autónoma de México-Unidad Zihuatanejo for providing logistic support in the field and for reference to specialized literature. We are also grateful to the UMAR for administrative and logistic support. Finally, we are grateful to Dr. Florian Weinberger and both anonymous reviewers who have helped to improve the quality of the article.
- Amado-Filho GM, Maneveldt GW, Pereira-Filho GH, Manso RCC, Bahia RG, Barros-Barreto MB, Guimaraes SMPB (2010) Seaweed diversity associated with a Brazilian tropical rhodolith bed. Cienc Mar 36:371–391View ArticleGoogle Scholar
- Bastida-Zavala JR, García-Madrigal MS, Rosas-Alquicira EF, López-Pérez RA, Benítez-Villalobos F, Meráz-Hernando A, Torres-Huerta AM, Montoya-Márquez A, Barrientos-Luján N (2013) Marine and coastal biodiversity of Oaxaca, México. Check List 9:329–390Google Scholar
- Chamberlain YM, Irvine LM (1994) Melobesioideae Bizzozero. In: Irvine LM, Chamberlain YM (eds) Seaweeds of the British Isles. Volume 1. Rhodophyta Part 2B Corallinales, Hildenbrandiales. HMSO, London, pp 159–234Google Scholar
- Dawson EY (1960) Marine red algae of Pacific Mexico. Part 3. Cryptonemiales, Corallinaceae subf. Melobesioideae. Pac Nat 2:3–125Google Scholar
- Foster MS (2001) Rhodoliths: between rocks and soft places. J Phycol 37:659–667View ArticleGoogle Scholar
- Fragoso D, Rodríguez D (2002) Algas coralinas no geniculadas (Corallinales, Rhodophyta) en el Pacífico tropical mexicano. Anales Inst Biol Univ Nac Autón México Bot 73:97–136Google Scholar
- Harvey AS, Woelkerling WJ (2007) Guía de identificación de rodolitos de algas rojas coralinas no geniculadas (Corallinales, Rhodophyta). Cienc Mar 33:411–426Google Scholar
- Henriques MC, Villas-Boas A, Riosmena-Rodríguez R, Figueiredo MAO (2011) New records of rhodolith-forming species (Corallinales, Rhodophyta) from deep water in Espírito Santo State, Brazil. Helg Mar Res 66:219–231View ArticleGoogle Scholar
- Huerta ML, Tirado J (1970) Estudio florístico-ecológico de las algas marinas de la costa del Golfo de Tehuantepec, México. Bol Soc Bot Méx 31:113–137Google Scholar
- León-Álvarez D, González-González J (1993) Algas costrosas del Pacífico Tropical. In: Salazar-Vallejo I, González EN (eds) Biodiversidad marina y costera de México. CONABIO y CIQRO, México, pp 456–474Google Scholar
- León-Tejera H, González-González J (1993) Macroalgas de Oaxaca. In: Salazar-Vallejo SI, González NE (eds) Biodiversidad Marina y Costera de México. CONABIO y CIQRO, México, pp 486–498Google Scholar
- Lobban CS, Tsuda RT (2003) Revised checklist of benthic marine macroalgae and seagrasses of Guam and Micronesia. Micronesica 35(36):54–99Google Scholar
- López-Pérez RA, Calderón-Aguilera LE, Reyes-Bonilla H, Carriquiry JD, Medina-Rosas P, Cupul-Magaña AL, Herrero-Pérezrul MD, Hernández-Ramírez HA, Áhumada-Sempoal MÁ, Luna-Salguero BM (2012) Coral communities and reefs from Guerrero, Southern Mexican Pacific. Mar Ecol 33:407–416View ArticleGoogle Scholar
- Masaki T (1968) Studies on the Melobesioideae of Japan. Mem Fac Fish Hokkaido Univ 16:1–80Google Scholar
- Mateo-Cid LE, Mendoza-González AC (2012) Algas marinas bentónicas de la costa noroccidental de Guerrero, México. Rev Mex Biodiv 83:905–928Google Scholar
- Mendoza-González AC, Mateo-Cid LE (1998) Avance de un estudio sobre las macroalgas marinas de Guerrero y Oaxaca. Ciencia y Mar 4:15–29Google Scholar
- Riosmena-Rodríguez R, Woelkerling WJ, Foster MS (1999) Taxonomic reassessment of rhodolith-forming species of Lithophyllum (Corallinales, Rhodophyta) in the Gulf of California, México. Phycology 38:401–417View ArticleGoogle Scholar
- Robinson NM, Hansen GI, Fernández-García C, Riosmena-Rodríguez R (2013) A taxonomic and distributional study of the rhodolith-forming species Lithothamnion muelleri (Corallinales, Rhodophyta) in the Eastern Pacific Ocean. Algae 28:63–71View ArticleGoogle Scholar
- Sneed ED, Folk RL (1958) Pebbles in the lower Colorado River, Texas; a study in particle morphogenesis. J Geol 66:114–150View ArticleGoogle Scholar
- Steller DL, Riosmena-Rodríguez R, Foster MS, Roberts CA (2003) Rhodolith bed diversity in the Gulf of California: the importance of rhodolith structure and consequences of disturbance. Aquat Conserv Mar Freshw Ecosyst 13:S5–S20View ArticleGoogle Scholar
- Steller DL, Riosmena-Rodríguez R, Foster MS (2009) Living rhodolith bed ecosystems in the Gulf of California. In: Johnson ME, Ledesma-Vázquez J (eds) Atlas of coastal ecosystems in the western Gulf of California. University of Arizona Press, United StatesGoogle Scholar
- Tsuda RT (2003) Checklist and bibliography of the marine benthic algae from the Mariana Islands (Guam and CNMI). Technical report. University of Guam Marine Laboratory 107: i–v, 1–49Google Scholar
- Wilks KM, Woelkerling WJ (1994) An account of southern Australian species of Phymatolithon (Corallinaceae, Rhodophyta) with comments on Leptophytum. Aust Syst Bot 7:183–223View ArticleGoogle Scholar
- Wilks KM, Woelkerling WJ (1995) An account of southern Australian species of Lithothamnion (Corallinaceae, Rhodophyta). Aust Syst Bot 8:549–583View ArticleGoogle Scholar
- Woelkerling WJ (1988) The coralline red algae: an analysis of the genera and subfamilies of nongeniculate Corallinaceae. University Press, LondonGoogle Scholar
- Woelkerling WJ (1993) Type collections of Corallinales (Rhodophyta) in the Foslie Herbarium (TRH). Gunneria 67:1–289Google Scholar
- Woelkerling WJ (1996) Subfamily Melobesioideae. In: Womersley HBS (ed) The Marine Benthic Flora of Southern Australia—Part IIIB. Gracilariales, Rhodymeniales, Corallinales and Bonnemaisoniales. Australian Biological Resources Study, Canberra, pp 164–210Google Scholar
- Woelkerling WJ, Irvine LM, Harvey AS (1993) Growth-forms in non-geniculate coralline red algae (Corallinales, Rhodophyta). Aust Syst Bot 6:277–293View ArticleGoogle Scholar