Table 2 Primers used to amplify sequences of mitochondrial and nuclear genes of Stegastes species

16S

L1987 (5′-GCC TCG CCT GTT TAC CAA AAA C-3′)

H2609 (5′-CCG GTC TGA ACT CAG ATC ACG T-3′)

[65]

COI

COI-FishF2 (5′-TCG ACT AAT CAT AAA GAT ATC GGC AC-3′)

COI-FishR2 (5′-ACT TCA GGG TGA CCG AAG AAT CAG AA-3′;

[66]

CytB

FishcytB-F (5′-ACC ACC GTT GTT ATT CAA CTA CAA GAA C-3′)

CytBI-5R (5′-GGT CTT TGT AGG AGA AGT ATG GGT GGA A-3′)

[67]

rhod

Rod-F2w (5′-AGC AAC TTC CGC TTC GGT GAG AA-3′)

Rod-R4n (5′-GGA ACT GCT TGT TCA TGC AGA TGT AGA T-3′)

[67]

rag1

RAG1-F3 (5′-GCC TCA GAA AAC ATG GTG CT-3′)

RAG1-R3 (5′-CCA CAC AGG TTT CAT CTG GA-3′)

[68]