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Table 2 Primers used to amplify sequences of mitochondrial and nuclear genes of Stegastes species

From: A reappraisal of Stegastes species occurring in the South Atlantic using morphological and molecular data

Gene Primers Tm (°C) References
16S L1987 (5′-GCC TCG CCT GTT TAC CAA AAA C-3′)
H2609 (5′-CCG GTC TGA ACT CAG ATC ACG T-3′)
52 [65]
COI COI-FishF2 (5′-TCG ACT AAT CAT AAA GAT ATC GGC AC-3′)
COI-FishR2 (5′-ACT TCA GGG TGA CCG AAG AAT CAG AA-3′;
50 [66]
CytB FishcytB-F (5′-ACC ACC GTT GTT ATT CAA CTA CAA GAA C-3′)
CytBI-5R (5′-GGT CTT TGT AGG AGA AGT ATG GGT GGA A-3′)
52 [67]
rhod Rod-F2w (5′-AGC AAC TTC CGC TTC GGT GAG AA-3′)
Rod-R4n (5′-GGA ACT GCT TGT TCA TGC AGA TGT AGA T-3′)
60 [67]
rag1 RAG1-F3 (5′-GCC TCA GAA AAC ATG GTG CT-3′)
RAG1-R3 (5′-CCA CAC AGG TTT CAT CTG GA-3′)
50 [68]
  1. Tm melting temperatures