- Original Article
- Open Access
A reappraisal of Stegastes species occurring in the South Atlantic using morphological and molecular data
Helgoland Marine Research volume 70, Article number: 20 (2016)
The taxonomic status of Pomacentridae species can be difficult to determine, due to the high diversity, and in some cases, poorly understood characters, such as color patterns. Although Stegastes rocasensis, endemic to the Rocas atoll and Fernando de Noronha archipelago, and S. sanctipauli, endemic to the São Pedro and São Paulo archipelago, differ in color pattern, they exhibit similar morphological characters and largely overlapping counts of fin rays and lateral-line scales. Another nominal insular species, S. trindadensis, has recently been synonymized with S. fuscus but retained as a valid subspecies by some authors. Counts and morphometric analyses and mitochondrial DNA (COI, 16SrRNA, CytB) and nuclear DNA (rag1 and rhodopsin) comparisons of three insular species (S. rocasensis, S. sanctipauli and S. trindadensis) and three other South Atlantic species (S. fuscus, S. variabilis and S. pictus) were carried out in the present study. Analyses of the principal components obtained by traditional multivariate morphometry indicate that the species in general have similar body morphology. Molecular analyses revealed conspicuous similarity between S. rocasensis and S. sanctipauli and between S. trindadensis and S. fuscus and a clear divergence between S. variabilis from Northeast Brazil and S. variabilis from the Caribbean region. Our data suggest that S. sanctipauli is a synonym of S. rocasensis, support the synonymy of S. trindadensis with S. fuscus, and reveal the presence of a likely cryptic species in the Caribbean that has been confused historically with S. variabilis.
Genetic analyses have revealed a large number of cryptic species in different groups of fish (e.g., [1, 2]). The term “cryptic” species, although the definition is not unanimous, generally refers to two or more distinct species that are nominally classified as one due to the difficult-to-distinguish morphologies . Morphological variations with no genetic differences have been explained as phenotypic plasticity, recent isolation, incomplete limits between species, or the product of selection . On the other hand, so-called polytypic species, in which genetically similar individuals of one species display conspicuously different coloration or morphology, occur more frequently [4, 5].
Inconsistencies between color patterns with morphology and genetic markers are relatively common and have been reported for a number of fish families including Pomacentridae [6–8]. This family, whose members are known as damselfishes, is one of the most represented marine groups in reef environments . Indeed, damselfishes are a diverse group, with 399 species , whose taxonomic definition is complicated by the occurrence of species complexes and marked variations in coloration patterns among individuals and geographic areas [11–14].
The western Atlantic contains Pomacentridae representatives of the genera Abudefduf (1 sp.), Microspathodon (1 sp.), Chromis (5 spp.), and Stegastes (7 spp.) [13, 15–19]. Some Stegastes species are considered endemic of insular environments of the South Atlantic, such as S. rocasensis Emery 1972, in the Rocas atoll (RA) and Fernando de Noronha archipelago (FNA), and S. sanctipauli Lubbock and Edwards 1981, exclusive to St. Paul’s Rocks. The first two oceanic formations are part of an alignment of submarine volcanoes that make up the Fernando de Noronha Chain [20, 21]. More southward, in the Trindade and Martim Vaz archipelago (TI), located in the easternmost part of the submarine mountain chain called Vitoria-Trindade and Martim Vaz, 1167 km off the coast of Espírito Santo State, southeastern Brazil, S. trindadensis Gasparini, Moura and Sazima 1999, was described and considered endemic to TI [20, 21].
Extensively overlapping morphometric characters in Stegastes (including the synonyms Brachypomacentrus Bleeker and Eupomacentrus Bleeker), a morphologically conservative group , in addition to cytogenetic  and dominant marker similarities , reveal uncertainty regarding the taxonomic status and phylogenetic relationships of S. rocasensis, S. sanctipauli and S. trindadensis, and the last was recognized as a synonym of S. fuscus Cuvier 1830 by Gasparini and Floater , Carter and Kaufman  and Pinheiro et al. . Based only on morphological criteria, S. rocasensis and S. sanctipauli have been considered similar to each other and to S. variabilis Castelnau 1855 [22, 27]. Although scarce biological information is available [14, 28, 29], there is some genetic data or information on its evolutionary history [23, 30, 31]. In order to clarify the taxonomic status and relationships of these three insular species and test the current hypotheses of ancestrality [22, 27], counts comparisons of serial elements, traditional and geometric multivariate morphometric analyses, and analyses of mitochondrial (16S ribosomal RNA—16S, cytochrome oxidase subunit 1—COI, and cytochrome B—CytB) and nuclear gene sequences (nuclear recombination-activating gene 1—rag1 and rhodopsin—rhod) were performed.
Individuals of Stegastes fuscus [MZUEL 8958, 22 specimens, 43.9–65.0 mm SL; coast of Rio Grande do Norte state (RN)—5°16′S, 35°22′W]; S. rocasensis (MZUEL 8956, 9, 36.6–66.5 mm SL; RA—3°51′S, 33°49′W); S. rocasensis (MZUEL 8960, 18, 42.7–78.2 mm SL; FNA—3°50′S, 32°24′W); S. sanctipauli [MZUEL 8959, 18, 58.3–80.1 mm SL; São Pedro and São Paulo archipelago (SPSPA)—0°55′N, 29°20′W]; S. trindadensis [MZUEL 13908, 12; Trindade island (TI)—20°30′S, 29°19′W] and S. variabilis (MZUEL 8957, 18, 33.3–57.2 mm SL; coast of RN—5°16′S, 35°22′W) were analyzed (Figs. 1, 2). Fragments of muscle tissue or fins were removed and stocked in microtubes (1.5 ml) with absolute ethanol and stored at −20 °C. Voucher specimens were fixed in 10 % formaldehyde, preserved in 70 % ethanol and deposited in the Museu de Zoologia from Universidade Estadual de Londrina (MZUEL—Table 1).
Counts, traditional and geometric multivariate morphometry
Injury-free adults of the species identified as Stegastes fuscus (n = 31); S. variabilis (n = 18), S. rocasensis (n = 5; RA); S. rocasensis (n = 18; FNA); S. sanctipauli (n = 17); and S. trindadensis (n = 26) were used in morphometric analyses. Counts of branched rays in dorsal, pectoral and anal fins and lateral-line scales were obtained for all the specimens.
Multivariate morphometry based on measuring linear distances made it possible to investigate variations among species of Stegastes, compared by Principal Component Analysis (PCA) , using SHEAR software . Sixteen measurements were taken with a digital caliper: standard length, head length, snout length, orbit diameter, greatest body depth, predorsal length, length of last dorsal-fin spine, length of longest dorsal ray, anal-fin base length, length of second anal-fin spine, length of longest anal ray, pectoral-fin length, pelvic-fin length, caudal-fin length, caudal-peduncle depth, and interorbital width.
Geometric morphometrics analyses were used to explore the variation in body shape in morphospace. Left side view photographs of the specimens were taken with an 8.1 megapixel Sony H10 camera, at a standard distance and position. Nine landmarks were digitized: (1) distal extremity of the premaxillary bone; (2) origin of dorsal fin; (3) end of dorsal fin; (4) origin of the anal fin; (5) end of the anal fin; (6) origin of the ventral fin; (7) insertion of pectoral fin; (8) posterior and (90 anterior orbit using tpsDig v2.16 software  and the images were ordered into a single TPS format with tpsUtil software . Next, Procrustes superimposition , Multivariate Analysis of Variance (MANOVA) and Canonical Variables Analysis (CVA) were carried out. Deformation grids were obtained from the canonical variables that most influenced morphological variation, in order to more clearly identify vector variations between species.
DNA extraction, PCR amplification and DNA sequencing
Molecular analyses involved extracting DNA from three individuals of Stegastes fuscus, S. variabilis, S. pictus, S. sanctipauli, and S. trindadensis and six of S. rocasensis (three from Rocas atoll and three from Fernando de Noronha archipelago).
Total DNA was extracted from muscle tissue according to proteinase K/phenol–chloroform protocols  Fragments of three mitochondrial genes (16S, COI, and CytB) and two nuclear genes (rag1 and rhod) from each individual were amplified using the polymerase chain reaction method (PCR). The PCRs were prepared to a final volume of 25 μl containing: 1.0 μl of total DNA (50 ng/μl), 3.0 U of Taq polymerase, 1.0 μl of 50 mM MgCl2, 1.0 μl of 10× buffer, 1.0 μl of 10 mM dNTP (deoxyribonucleotide triphosphate), 1.0 μl of each primer (10 μM), and ultrapure water to complete the final volume of the reaction. The thermal profiles for PCR were as follows: 95 °C for 5 min, followed by 35 cycles at 95 °C for 30 s, annealing temperature for 45 s (Table 2), and 72 °C for 45 s, with final elongation at 72 °C for 7 min.
The PCR products (5 μl) were purified with exonuclease I enzymes (3.3 U/reaction) and shrimp alkaline phosphatase (0.66 U/reaction) (GE Healthcare), in a thermal cycler, submitting the mix to a 30-min cycle at 37 °C and 15 min at 80 °C. The samples were sequenced in ACTGene Análises Moleculares Ltda. through the ABI-PRISM 3500 Genetic Analyzer automatic DNA sequencer (Applied Biosystems).
Analysis of sequences
The electroferograms obtained were verified using BioEdit software . The sequences of each gene were aligned using MUSCLE  in the MEGA 6 software package . The sequences of mitochondrial (16S, COI and CytB) and nuclear genes (rag1 and rhodopsin) from each individual were concatenated, forming a single nucleotide sequence. Sites with gaps were excluded from all genetic analyses.
Sequences of the COI gene of 22 additional Stegastes species or populations were obtained from BOLD (www.boldsystem.org) and used for the comparative analyses. The mean genetic divergence rates (Kimura-2-parameter model) and the neighbor-joining tree were obtained with the MEGA 6 software .
The relationships among insular species (S. sanctipauli, S. rocasensis and S. trindadensis) were determined by the Maximum Likelihood Method using MEGA 6 software . The best evolutionary method for the sequences was determined by jModelTest2 software  using the Akaike information criterion. Macrodon ancylodon (Sciaenidae) and Haemulon aurolineatum (Haemulidae) were used as outgroups. These families are outgroup candidates because they, along with the Pomacentridae, are members of order Perciformes [10, 42]. The number of polymorphic sites along the sequences was estimated using DnaSP 5 software , while mean genetic divergence rates (p-distances) and the mean number of intra and interspecific nucleotide differences were estimated by MEGA 6 software .
Quantification of fin rays and lateral-line scales
Frequency distributions of counts of fin rays and lateral-line scales are presented in Table 3. There is great similarity among species, except for S. pictus, which modally exhibits fewer pectoral-fin rays than the other species. Numerical differences were observed between counts taken in populations of S. variabilis from Brazil and those described for Caribbean region . Counts do not discriminate among S. fuscus, S. variabilis, S. rocasensis and S. sanctipauli.
Principal Component Analysis (PCA)
The first principal component retained 88.6 % of data variance, whereas in the second and third axes (sPC2 and sPC3), it was 2.6 and 2.1 %, respectively (Table 4). The first PC is positively related to all measurements and was interpreted as being related to size, while the second and third, with positive and negative values, respectively, represent the shape. The individual scores for the species revealed partial overlapping among them in these components (Fig. 3). However, in the second axis, S. variabilis was completely separated from S. rocasensis, S. trindadensis and S. sanctipauli, but with greater similarity to S. fuscus, whereas S. fuscus presented intermediate scores between these species. The individuals identified as S. sanctipauli and S. rocasensis are not separated morphometrically along sPC2 and sPC3. In the third axis S. trindadensis is separated from S. rocasensis (Fig. 3). The samples identified as S. rocasensis from Fernando de Noronha archipelago and S. rocasensis from Rocas atoll are not separated in any of the axes, nor is the sample of S. sanctipauli.
The variables that discriminate S. fuscus and S. variabilis from S. rocasensis, S. trindadensis and S. sanctipauli by the second PC are caudal peduncle depth, interorbital width and body depth (positive loadings, Table 4). By contrast, the variables with the highest values in S. rocasensis, S. trindadensis and S. sanctipauli are length of the longest anal ray, length of the second anal spine, snout length, and length of the longest dorsal ray (negative loadings in Table 4). Stegastes trindadensis differs from both populations of S. rocasensis by having a longer pelvic fin, last dorsal spine, anal-fin base, and longest dorsal ray (positive loadings, Table 4), and shorter snout, smaller orbit diameter, and shorter head (negative values, Table 4).
Geometric morphometric analysis
Canonical variables 1 and 2 contributed more to the shape variation among samples of Stegastes (Procrustes MANOVA: SS = 0.086; df = 70; F = 11.93; p < 0.001; Pillai’s trace = 2.37; Pillai’s trace p < 0.001), accounting for 83.7 % (CV1 = 57.3; CV2 = 26.4; p < 0.001). The individual scores of the species demonstrate a marked overlap in the morphospace of S. fuscus and S. variabilis along the two axes. Stegastes sanctipauli individuals overlapped with those of S. rocasensis (RA; FNA) (Fig. 4). Stegastes rocasensis individuals of RA and FNA were more discriminated between each other than with the sample of SPSPA. The deformation grids obtained from CV2 show morphological distinctions between S. fuscus and S. variabilis relative to landmarks 1–3 and 6–9, which are related to the position of the fins (anal, dorsal, pelvic and pectoral), mouth and eyes (Fig. 4a). The warped outlines generated from CV1 data demonstrate the morphological diversification of S. rocasensis and S. sanctipauli in relation to the position of landmarks 2–7 (Fig. 4b), which correspond primarily to variations in anterior and posterior body height. Body variations between S. fuscus and S. trindadensis show differences in pectoral- and anal-fin position, as well as body length and height (Fig. 4c).
For genetic diversity analyses 90 nucleotide sequences were generated, 18 for each of the five molecular markers analyzed. The access numbers of sequences in GenBank are listed in Table 1. After the alignments the sequences resulted in 540 base pairs (bp) of gene 16S, 668 bp of CytB, 633 bp of COI, 290 bp of rag1 and 449 bp of rhodopsin. The concatenation of five fragments resulted in consensus sequences of 2580 bp, which were used in genetic comparisons.
Stegastes sanctipauli (SPSPA) and S. rocasensis (FNA/RA) specimens shared identical haplotypes for all the genes, except for one specimen of S. rocasensis from the FNA, which exhibited a mutation (ts G → A) in the CytB gene. Stegastes variabilis showed more genetic differences in relation to S. rocasensis, S. sanctipauli and S. trindadensis than to the coastal species S. fuscus. The mean number of intraspecific nucleotide differences ranged from 0 to 2 (Table 5). The nucleotide diversity of concatenated sequences was extremely low in S. rocasensis from FNA and RA, and absent in S. sanctipauli and S. rocasensis from RA (0 ± 0.0). The highest mean nucleotide differences in the species under study was found between S. pictus and S. variabilis (228 ± 13).
Comparative genetic analyses
A neighbor-joining analysis of the COI sequences with 27 species/populations shows that the genetic distance among species ranges from 0.000 ± 0.000 to 0.207 ± 0.021 (Additional file 1: Table S1). Genetic distance values of 0.000 ± 0.000 were observed between Stegastes rocasensis from literature and present study, between S. rocasensis and S. sanctipauli, and between S. pictus from literature and present study. A genetic distance of 0.001 ± 0.001 was observed between S. trindadensis and a sample of S. fuscus analyzed in the present study. Between S. variabilis and a sample of S. fuscus analyzed in the present study a genetic distance of 0.040 ± 0.008 was observed. The relationship among samples is showed in the Fig. 5.
Maximum likelihood using the concatenated sequences of 16S, COI, CytB, rag1 and rhod genes produced resolved clusters with high bootstrap values that clarify the relationships among Atlantic species of Stegastes (Fig. 6). In both analyses (COI and concatenated sequences) the data clearly demonstrate a clade formed by S. rocasensis (RA and FNA) and S. sanctipauli, whose individuals are genetically the same, as well as almost complete genetic similarity between S. fuscus and S. trindadensis. Stegastes variabilis and S. pictus show a low genetic similarity with insular forms.
Morphological approaches, associated with mitochondrial- and nuclear-gene analyses between continental and insular species of Stegastes in the western Atlantic displayed putative incongruities with respect to the taxonomic status attributed to the insular species S. rocasensis, S. sanctipauli, and S. trindadensis.
Classification of Stegastes species is particularly dependent on color patterns, given that they exhibit conservative morphology . Indeed, discrimination of some species through the exclusive use of phenotypic criteria has been problematic , since it reveals limited or ambiguous morphological characters or those with significant plasticity. Furthermore, phenotypic variations are not always effective phylogenetic characters, given that they may reflect adaptations to different ecological regimes to which the species are subjected in their distribution area .
Aspects of coloration have masked the marked morphological similarities between Stegastes rocasensis and S. sanctipauli as observed in the present study. The juveniles and adults of the former are notably bicolored (blue dorsally and yellow ventrally), exhibiting dark lines on the side of the body in large individuals. Stegastes sanctipauli exhibits bright yellow to dark green or yellowish-brown coloration in adults, whereas juveniles have yellow heads and bodies, darkening slightly on the dorsal portion. Dark lines are observed on the flanks below the lateral line. The presence of a body with yellow color patterns is also shared with S. variabilis. On the other hand, juvenile Stegastes fuscus exhibits bright-blue coloration on the dorsum, becoming lighter ventrally, and adults are largely brown. Stegastes trindadensis displays similar coloration to that of the continental species S. fuscus, from which it is distinguished in the juvenile phase by the yellow coloration of the beginning of the dorsal fin .
In the Fernando de Noronha archipelago, Rocas atoll and São Pedro and São Paulo archipelago, the abundance of S. rocasensis and S. sanctipauli individuals is directly related to the structural composition of the environment and diversity of the substrate cover . In this respect, these insular regions can mobilize phenotypic responses adaptive to different selective pressures, both in terms of body shape and coloration of individuals from each region. Abnormal body coloration has been described in individual species of Stegastes [48, 49], including four of those analyzed here: S. fuscus, S. variabilis, S. rocasensis and S. pictus [14, 15, 50]. These variations can be due to non-genetic factors , or even, in this or other genera, intrapopulational characteristics, without being necessarily supported, however, by very high levels of genetic differentiation .
Menezes and Figueiredo  underscored the greater similarity in coloration and morphological characters between S. fuscus, S. rocasensis and S. sanctipauli, compared to other species of Stegastes on the Brazilian coast. Indeed, the modal values of counts in the three species are largely concordant, with marked overlapping of minimum and maximum values. However, this did not occur with S. pictus, which show a lower value for pectoral rays (18).
Analyses of the principal components obtained by traditional multivariate morphometry indicated that the species in general have similar body morphology. In relation to insular forms, S. rocasensis from RA and FNA exhibit overlapping morphological aspects. These two populations of S. rocasensis demonstrated markedly similar morphology to that of S. sanctipauli, and accurate ordering on the sPC2 and sPC3 axes. In fact, both species display morphological patterns more similar to each other than to the other species of Stegastes analyzed. On the other hand, the continental species S. variabilis exhibits only partial discrimination of insular individuals, which could suggest the effect of different environmental pressures.
Although morphological diversification occurs between Stegastes species, they share conservative body patterns. Similarly to multivariate analyses, geometric morphometry analyses also show significant morphological similarity between S. rocasensis (FNA) and S. sanctipauli. Curiously, subtle body differences were more common between S. rocasensis individuals from RA and FNA, than with S. sanctipauli. This may be caused by distinct environmental factors, causing a disruptive selection of morphotypes adapted to local conditions. Indeed, as a response to environmental characteristics, many fish species demonstrate considerable phenotypic plasticity that increases their aptitude to a determinate environment . When compared to other insular ecosystems, the low habitat diversity of Rocas atoll  may have driven the morphological changes observed in the population of S. rocasensis from RA due to the different ecological resources in the systems [46, 55, 56].
Variations in body shape between the species of Stegastes analyzed were significant in terms of the positions of the dorsal and anal fins, and body height. These ecomorphological divergences suggest adaptations related to the swimming process, with direct implications on habitat use , biotic interactions  and foraging . On the other hand, morphological changes in position and mouth size could reflect different degrees of food specialization, especially in relation to the type and potential size of the prey . The phenotypic plasticity identified in populations of S. rocasensis from RA, FNA and SPSPA indicate an intense environmental effect on the local adaptive patterns of each population. Indeed, in the damselfish Abudefduf saxatilis, counts and multivariate morphometry analyses between different populations, revealed clinal variation between northeastern/RA-SPSPA regions and the southern coast of Brazil, in addition to marked morphological differences between the populations of Rocas atoll, Fernando de Noronha archipelago and São Pedro and São Paulo archipelago . This condition has been identified in other Atlantic species, such as the frillfin goby (Bathygobius soporator), which exhibits significant morphological variations between contiguous populations along the Brazilian coast, the action of ecological factors being the likely mobilizers of morphological diversification .
In groups with marked phenotypic plasticity, such as Pomacentridae, genetic analyses are particularly revealing regarding population structuring , or defining the biological status of groups whose morphological characteristics have not been resolved. Analyses of 16S, COI, CytB, rag1 and rhod gene sequences, widely used in phylogenetic evaluation, enabled the assessment of taxonomic status and the level of genetic divergence between the insular forms S. rocasensis, S. sanctipauli and S. trindadensis in relation to other congeneric species. In this respect, despite the various sequences used, S. rocasensis and S. sanctipauli showed no genetic divergence between them, suggesting that they represent a single species. Similarly, a very low genetic divergence was identified between S. fuscus and S. trindadensis (0.1 %). Our genetic data support the synonymy of S. trindadensis with S. fuscus and do not support subspecific division within that species.
Analyses with the COI sequences of Stegastes species confirm that samples of S. rocasensis (our data and also data from the literature) and S. sanctipauli are genetically identical. The genetic distance between our samples of S. trindadensis and S. fuscus also show that they are almost identical. A single record in the BOLD database identified as S. fuscus does not match our sequences of S. fuscus but matches sequences of S. diencaeus and appears to be a misidentification. A comparison between our data for S. pictus and the BOLD data shows no difference between them. On the other hand, a comparison of our COI data of samples of S. variabilis from northeast Brazil with sequences of S. variabilis from the Caribbean area showed a genetic distance between them of d = 0.040 ± 0.008. Barcoding studies with fishes showed that the genetic distance (based on the Kimura-2-parameter model) between species usually is around 2 % ; thus, the present data on S. variabilis suggest the possible occurrence of a cryptic species. Although counts of more Brazilian specimens of S. variabilis are needed, Table 3 indicates that there may be modal differences in some counts between Brazilian and Caribbean populations.
Stegastes sanctipauli was described as endemic to SPSPA, an insular region approximately 600 km from FNA and 700 km from RA, an occurrence site of S. rocasensis. The occurrence of S. rocasensis in the remote SPSPA  can indicate color polymorphisms or evidence of sporadic recruitment between these two areas. Nevertheless, it demonstrates a more geographically or morphologically diffuse situation than was previously believed. Thus, the evidence that S. rocasensis and S. sanctipauli are the same species suggests the occurrence of color polymorphism maintained by different selective pressures between these insular regions. Recent evidence of phylogeographic patterns in the Brown Chromis (Chromis multilineata) demonstrates gene flow, involving FNA and SPSPA . Despite the suggestion of greater isolation in the SPSPA population, also identified for S. sanctipauli , the population of this species exhibits moderate genetic structuring between the insular regions and other coastal populations. Our morphological and genetic data show that the physical distance between the sites of S. rocasensis and S. sanctipauli apparently do not preclude gene flow between these occurrence areas.
It cannot be ruled out that S. rocasensis, S. sanctipauli and S. fuscus from Trindade Island (S. trindadensis) may represent species in statu nascendi , where reproductive isolation remains incomplete, but the absence of genetic differentiation and distinct morphological characters between S. rocasensis and S. sanctipauli and between S. trindadensis and S. fuscus is strong evidence for the recognition of two versus four species. On the other hand, the differentiation observed between Brazilian and Caribbean S. variabilis is strong evidence of a putative new species.
Fernando de Noronha archipelago
São Pedro and São Paulo archipelago
Trindade and Martim Vaz archipelago
16S ribosomal RNA
cytochrome oxidase subunit 1
- Rag1 :
nuclear recombination-activating gene 1
- Rhod :
Museu de Zoologia from Universidade Estadual de Londrina
Principal Component Analysis
Multivariate Analysis of Variance
Canonical Variables Analysis
polymerase chain reaction
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ASS, RSR, and WFM conceived the study. ASS and RXS collected the specimens. ASS, CO and WFM carried out the molecular genetic analyses. RSR and OAS carried out the counts and the specimens identification. OAS, PAL-F, RXS and WFM carried out the morphometric analyses. ASS and WFM wrote the manuscript, with significant input of all coauthors. All authors read and approved the final manuscript.
The authors thank the National Counsel of Technological and Scientific Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico) (CNPq—Proc. 141234/2009-1; 309879/2013-2; 442664/2015-0) for financial support, and for the research scholarship awarded to ASS and RXS, and Instituto Chico Mendes de Conservação da Biodiversidade for the specimen collection license (#19135-5).
The authors declare that they have no competing interests.
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All the authors approved the paper for publication in this journal.
Ethics approval and consent to participate
The experimental work fulfills all ethical 331 guidelines regarding the handling of specimens. The collection and handling of specimens followed protocols approved by the Ethics Committee on the Use of Animals of the Federal University of Rio Grande do Norte (Process 044/2015). All the authors consent in participate and are in agreement with the article content.